The Analyst GT is equipped with 20 plate magazines in a bi-directional stacker. The plate is set up for reading and images are acquired according to specified parameters. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. For more information, please visit us at microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. The SpectraMax ® i3x. SpectraMax Spectrofluorometers. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug. Read More. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Features. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. 1. Products : SpectraMax M2/M2e reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, Gemini EM reader, SpectraMax Plus 384 reader. Molecular devices SpectraMax M5 Pdf User Manuals. Description 1. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. 2. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Set up Identify Acquire Analyze Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. This five-mode microplate reader with automatic filter identification. Choose from a three- (M3), four-. There is currently no charge for using this plate reader. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Reader Documentation Version No. The dedicated luminescence PMT has extremely low background counts and superior performance in the blue wavelengths. 0200-0729. 010 OD 2. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. 58% for the detection module and 2. Model: SpectraMax M5. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Supported Plates and Cuvettes. The SpectraMax® iD3 Multi Mode Microplate . The SoftMax® Touch Software is installed in. 0 . 304 signal level saturation. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. The chamber. 3: The cuvette chamber. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Compatible Fluorescence Polarization Plate Readers. com 4. used. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Microplate Readers. N. Microplate Readers Biotek Epoch Microplate Reader. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Other technologies such as imaging. Certain functionalities in the table above requires specific configurations. 12. 87. #3635 or Greiner cat. See SpectraMax® Microplate Reader User Manual Download Page. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. 8. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. These lab workhorses are used in drug discovery. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. . Spectramax M5. Read More. 2. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Temperature Regulation. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. What plates do you recommend for SpectraMax Plate Readers? Luminescence : Solid white plates for top reads and white plates with clear bottoms for. Perform the scan and note the wavelength that gives maximum emission. Maximum signal with lowest background. The optical systems . 0 out of 5 $ 9,995 $ 9,495. Instrument setup and sample analysis. 9. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Prepare your iD series instrument for shipment. SpectraMax M5 microplate reader. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. NIST-traceable solution for validating the SpectraMax 340PC384 system. The SpectraMax® ABS Plus Microplate Reader can run . The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading STAKMAX. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. This Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. If the computer is connected to the Internet: Select Search Automatically for updated driver software. We would like to show you a description here but the site won’t allow us. Put the plate in the SPECTRAmax PLUS drawer, then click the Read but-ton in the Plate section’s Status Bar. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Supersedes 1. These robust readers have been placed in labs from Antarctica to. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. Reader Documentation Version No. SpectraMax M3, M4, M5, and M5 e. The reader has the most features and capabilities in one unit. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. com 4. Figure 1. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . 0 . The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. IMAP assays are based on binding of. We specialize in many of the Molecular Devices microplate readers, washers, and stackers including the SpectraMax®, VersaMax™, Gemini™, FlexStation™, AquaMax®, and StackMax® lines. Pathcheck Pathlength Measurement Technology. The plate is set up for reading and images are acquired according to specified parameters. We empower scientists to advance discovery Automated, end-to-end solutions span research disciplines to help scientists discover, scale, and industrialize biology. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. No accessories are needed and SoftMax® Pro Software. 3: The cuvette chamber. iD5. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Choose from a three- (M3), four- (M4), orThey include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate. book Page 21 Wednesday, July 7, 2010 2:09 PM. When the user applies a set temperature of 37°C, the top is heated to 37. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. 315 can't find zero order. Wide range of applications. To assess the sensitivity of each microplate reader, a plate was set up with 32 wellsThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. spr SpectraMax Plus 384. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. *This plate definition in SoftMax Pro Software is the same for black and white plates. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Page 28 Chapter 5: Read Modes and Read Types 1. series measured in a 96-well plate in the SpectraMax M5 and the Analyst GT. 0 out of 5 $ 3,799. Our lab regularly left plates with aqueous buffer in them in the plate reader (service contract lapsed for several years. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Detection of pro-inflammatory factorsThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Test up to 3. 3 or greater. SpectraMax L reader, SpectraMax M5/M5e reader What is the StakMax timing with SpectraMax Plate Readers? If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the StakMax are as follows:. +44 118 944 8000. View the Molecular Devices website. Human macrophages choreograph tissue development. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. easy to use and read multiple plates very quickly. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 VAL014, right table) assays on the SpectraMax M5, M5e, or FlexStation 3 reader are shown below. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. 6 explains the lower values on the LmaxII384 plate reader. Weighs about - 23 lbs. Label-free detection. Most papers they cultured bacteria to OD600>0. Here the delta mP was 336, and Z’ factor was 0. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). plate analysis, custom calculations, and personalized reporting. Figure 1. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 1. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. . 1. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Step 4. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 12. Call 516-939-0888 to check. The M5e Reader is easily automated. Reader Documentation Version No. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. " SpectraMax plate reader is very easy to use, with detection of absorbance, fluorescence, luminescence, etc. Click SoftMax Pro Software User Guide. Rating: 5. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 100 µL of AK Reagent Working Solution was added to each well, and the plate was read within 30 minutes. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Only Molecular Devices microplate plate readers offer the capability to . 305 reference level saturation. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Manufacturer: Molecular Devices. SpectraMax M5 Multi-Mode Microplate Reader. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. Fluorescence intensity was measured using a. Information on how to reserve the plate readerThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Microplate Reader Shelf. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. EarlyTox Caspase-3/7 R110 Assay. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. Figure 1. The M5 can read volumes down to 2uL with the. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Description. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). 8. Update Driver. M5 E -HTRF Readers. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 2. Table 1. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orSPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. In an analogous manner, the same sample names were assigned to a CuvetteSet section. When the correct COM port is selected, the icon of the SpectraMax L reader in the upper left hand corner will be displayed in color. We would like to show you a description here but the site won’t allow us. free tracer decreases, resulting in an. Select the Driver tab. If no sound is heard during initialization, contact technical support. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. SpectraMax ABS Plus SpectraTest ABS2 v1. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SeePlateAdapteronpage20. After the plate is read, data will appear in the plate section: 11. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 00800 665 32860. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. 40266The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. eu@moldev. Our absorbance plate readers feature our PathCheck Sensor technology and. 5 pM (0. SeePlateAdapteronpage20. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. ScanLater Western Blot Detection Cartridge. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. The multi-mode. Prepare your i3/i3x instrument for shipment. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Italic and boldface type are used for emphasis. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. For prep samples treated with the ELISA kit reagents, it is fast and user friendly. Category 2 and 3 microplate readers use gradient heating as well. Information on how to reserve the plate readerThe GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. After the plate is read, data will appear in the plate. Tecan Infinite M1000 Microplate Reader w/ Injectors $10,000. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. com. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. SpectraMax M5e Multi-Mode Microplate Reader. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. The assay window spanned four orders of magnitude. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Manufacturer: Molecular Devices Model: SpectraMax Plus 384 The SpectraMax ® Plus 384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. SpectraMax Paradigm Multi-Mode Safety Guide SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Fluorescence Microplate Reader Comparison Chart. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Our 15 years of experience means we understand these instruments as well as the manufacturer, and it allows us to provide top-quality service. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Molecular Devices. The dual monochromator optics allow the. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Be sure to insert . Uncontrolled Copy for Training and Reference Purposes Only . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. 000 to 4. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Five. Please contact Tatiana to reserve the plate reader time: tnl1@psu. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The SpectraMax M5 Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. White plates reflect the light, enabling even low levels of Luminescence to be detected. 40266 Email: drugdiscoverytech@lifetech. 3) At the bottom of the strip, click on "Show. 01) + 0. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Parts Only. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. 25 pM (0. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Turn the reader on and secure the USB cable. The instrument settings were identical to those used in Example 1 above. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orSetup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 40266 Email: drugdiscoverytech@lifetech. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. 11. Thank you!. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Self Service Available. The FlexStation 3 reader’s optics are designed to easily adapt to changes in assay requirements while maintaining the optimal performance of a single-mode reader. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Description 1. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 200 ‚ 1000 nm. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. With optimized reagents, option to operate the. ”The SpectraMax 190 reader can be integrated with Molecular . 00. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Five preferred detection modes. Uncontrolled Copy for Training and Reference Purposes Only . 2. An integration time of 1 second was used for each reader. The BD plate dimensions are 5. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and auction. The guide also covers the software features, the specifications, and the accessories of the systems. 1. Find Molecular Devices SpectraMax M3/M4/M5/M5e Only Molecular Devices microplate plate readers offer the In need of a service manual for the SpectraMax M5 Find Molecular Devices SpectraMax Plus 384 – Forums, 190 – 1000 nm, tunable Molecular Devices SpectraMax Plus 384 Plate Reader. 99. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates FL1, Gemini EM reader, Gemini XPS reader, SpectraMax M5/M5e reader My SpectraTest FL one plate is failing for LLD for the wet test. Description 1. Unplug the power cable from the back of the instrument. FL1 Validation Plate automatically validates the performance of the reader. Read Modes Normal and Sweep Absorbance Measurement Range 0. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Then the plate was returned to the. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Plate reader instructions Computer. . Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Molecular Devices SpectraMax Plus 384. Performing an enzyme dilution series verifies the enzyme and assay performance and allows calculation of the EC 80 concentration of enzyme that will be used for inhibitor screening assays. The Molecular Devices SpectraMax M5 plate reader is dual monochromator, allowing selection of any absorbance wavelength between 200 nm and 1,000 nm, and any excitation wavelength between 250 nm and 850 nm for reading in fluorescence intensity, time-resolved fluorescence (TRF), or wavelength-selectable luminescence modes, and 400 - 750 nm. SpectraMax M5 Multi-Mode Microplate Reader. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Fluorescein 0. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. We show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. Molecular Device SpectraMax M2 Plate Reader - Certified with Warranty. Information on how to. SpectraMax® Microplate Readers and SoftMax® Pro Software. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. For more information, please visit us at Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Supported Plates and Cuvettes. 0 out of 5 $ 5,999. Temperature Regulation. 2. 00. SpectraMax Injector Cartridge with SmartInject™ Technology. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read.